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CDRI Lucknow Develops DNA Gel Stain 'GreenR' Ending Dependency on Imported RT-PCR Test Kits

Written by : Dr. Aishwarya Sarthe

September 28, 2023

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'GreenR' is expected to reduce costs for life sciences researchers, finding applications in molecular diagnostics and facilitating PCR-based testing for various diseases.

The CSIR-Central Drug Research Institute (CDRI) in Lucknow has developed an indigenous DNA Gel stain named 'GreenR.' 

This development aims to eradicate India's dependency on imported dye critical for RT-PCR tests used in COVID-19 and various diagnostic examinations, including molecular diagnostics and other PCR-based disease testing.

The 'GreenR' stain is a collaborative effort between CDRI chief scientist Atul Goel and four researchers collaborating with Hyderabad-based Biotech Desk. In the past, India relied on other nations to import this dye, which was crucial for producing test kits. 

However, 'GreenR' reduces this dependency, offering a cost-effective alternative to the expensive RT-PCR diagnostic procedure and minimising the need for imports.

Dr. Kamal Saba, the R&D head at Biotech Desk and a former Ph.D. student of CSIR-IICT in Hyderabad, said, "We collaborated with CDRI to develop a dye equivalent to Cyber Dream, presently imported by India. We achieved this milestone during the COVID-19 pandemic in 2021. The dye is compatible with real-time PCR test kits, and we hold a patent for its production, which is already underway."

The collaboration has resulted in the capacity to produce 100mg of 'GreenR' per week, sufficient for conducting two lakh RT-PCR tests. Additionally, Biotech Desk's diagnostic kit is currently in the certification phase.

Talking about the company's portfolio, Biotech Desk, based in Hyderabad, Telangana, specialises in recombinant DNA technology, DNA libraries, metagenomics, protein crystallography, custom synthesis of building blocks, and custom and catalog antibodies. 

'GreenR' is expected to significantly reduce costs for life sciences researchers, finding applications in molecular diagnostics and facilitating PCR-based testing for various diseases.

This achievement aligns with the Indian government's 'Make in India' initiative to reduce the nation's reliance on foreign sources for critical healthcare supplies. 

'GreenR' ensures the availability of essential materials for diagnostic tests, bolstering India's position in the healthcare sector. This solution eliminates the need for importing crucial materials and offers a cost-effective alternative that can benefit researchers and healthcare professionals in India and beyond.


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